Anti-TRAF6 Rabbit pAb - 100 µL
   

Anti-TRAF6 Rabbit pAb - 100 µL

Antibodies-Oncology
AO-03-GB112201-100
  • Specification: 100 µL
  • Applications: WB | IHC/IF
  • Species: H,M,R | M,R
  • Dilution: 1:500-1:1000 | 1:400-1:800
  • Positive tissue: liver, brain | lung, substantia nigra
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Product Information

Protein full name

TNF receptor-associated factor 6

Synonyms

E3 ubiquitin-protein ligase TRAF6, RING-type E3 ubiquitin transferase TRAF6, Traf6, RNF85, RING finger protein 85, Interleukin-1 signal transducer

Uniprot ID

P70196

Immunogen

Recombinant protein corresponding to Mouse TRAF6

Isotype

IgG

Predicted MW./Observed MW.

60 kDa / 60 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000liver, brain
IHC/IFMouse, Rat1: 400-1: 800lung, substantia nigra

Background

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family.

Images

01rPMqDZWoInxBS.jpg

 WB analysis of TRAF6(GB112201).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02VGCyx4lBQmDbl.jpg

 WB analysis of TRAF6(GB112201).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

0368KePExMxSeIe.jpg

 IF analysis of TRAF6(GB112201)red.
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

04cfJJar2PrwtPj.jpg

 IF analysis of TRAF6(GB112201)red.
Sample: Mouse substantia nigra, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

05md2G14h6FEls5.jpg

 IF analysis of TRAF6(GB112201)red.
Sample: Rat lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 400, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB112201-100
: 10 Produits
Hurry! only 10 items left in stock.

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