Anti-IKB alpha Rabbit pAb - 100 µL
   

Anti-IKB alpha Rabbit pAb - 100 µL

Antibodies-Oncology
AO-03-GB11212-100
  • Specification: 100 µL
  • Applications: IHC | IF
  • Species: H | H
  • Dilution: 1:500-1:3000 | 1:500-1:1000
  • Positive tissue: lung cancer, liver cancer, stomach cancer | lung cancer, liver cancer, stomach cancer
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429,00 €
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Product Information

Protein full name

NF-kappa-B inhibitor alpha

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, Ikβ-α, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

Uniprot ID

Q9Z1E3

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse IKBα

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Applications

ApplicationsSpeciesDilutionPositive Tissue
IHC/IFHuman1: 500-1: 3000colon cancer, stomach cancer

Background

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Images

01MjOWvvq5tNIQg.jpg

 IHC analysis of IKB alpha(GB11212).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

025C1Qm9xpTqAYU.jpg

 IHC analysis of IKB alpha(GB11212).
Sample: Human stomach cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03DQfTIsIwJ8mVo.jpg

 IHC analysis of IKB alpha(GB11212).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

048x4FlE8RabQ8L.jpg

 IHC analysis of IKB alpha(GB11212).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

05eQ3UB8mbK8QyU.jpg

 IHC analysis of IKB alpha(GB11212).
Sample: Human stomach cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB11212-100
: 10 Produits
Hurry! only 10 items left in stock.

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