Anti-IKK alpha Rabbit pAb - 100 µL
   

Anti-IKK alpha Rabbit pAb - 100 µL

Antibodies-Oncology
AO-03-GB11292-2-100
  • Specification: 100 µL
  • Applications: IHC/IF
  • Species: H,M,R
  • Dilution: 1:200-1:1000
  • Positive tissue: brain, breast cancer, colon cancer, kidney, lung cancer, testis
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Product Information

Protein full name

Inhibitor of nuclear factor kappa-B kinase subunit alpha

Synonyms

Chuk, CHUK, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, Conserved helix-loop-helix ubiquitous kinase, Component of inhibitor of nuclear factor kappa B kinase complex

Uniprot ID

Q60680

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse IKKα

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm, Nucleus

Applications

ApplicationsSpeciesDilutionPositive Tissue
IHC/IFHuman, Mouse, Rat1: 200-1: 1000brain, breast cancer, colon cancer

Background

IKK-α is a member of the serine/threonine protein kinase family and forms a complex in the cell with IKK-βand NEMO. NF-κB transcription factors are normally held in an inactive state by the inhibitory proteins IκBs. IKK-α and IKK-β phosphorylate the IκB proteins, marking them for degradation via ubiquitination and allowing NF-κB transcription factors to go into the nucleus. Once activated, NF-κB transcription factors regulate genes that are implicated in many important cellular processes, including immune response, inflammation, cell death, and cell proliferation.

Images

013nMzMeS5hPhME.jpg

 IHC analysis of IKK alpha(GB11292-2).
Sample: Human lung cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 600, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

023foiyqVb4fF0g.jpg

 IHC analysis of IKK alpha(GB11292-2).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 600, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

03NaIT6l5acteN4.jpg

 IHC analysis of IKK alpha(GB11292-2).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min.
Primary antibody: 1: 600, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

04N2ic9ZwwPGBRd.jpg

 IF analysis of IKK alpha(GB11292-2)red.
Sample: Mouse kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

05772ynWAj6mRNR.jpg

 IF analysis of IKK alpha(GB11292-2)red.
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 98°C, 20min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 600, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

Datasheet: Download datasheet 1

Antibodies-Oncology
: AO-03-GB11292-2-100
: 10 Produits
Hurry! only 10 items left in stock.

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