Anti-IKK gamma/NEMO Rabbit pAb - 100 µL

AO-03-GB11628-100
  • Specification: 100 µL
  • Applications: WB
  • Species: H,M,R
  • Dilution: 1: 500-1: 1000
  • Positive tissue: lung, brain
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Product Information

Protein full name

NF-kappa-B essential modulator

Synonyms

IKBKG, AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9, IKKAP1, IKKG, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma, inhibitor of nuclear factor kappa B kinase subunit gamma

Uniprot ID

O88522

Immunogen

Recombinant protein corresponding to Mouse IKK gamma

Isotype

IgG

Predicted MW./Observed MW.

48 kDa / 48 kDa

Purity

Affinity purification

Applications

ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000lung, brain

Background

NF-kappa-B essential modulator (NEMO) also known as inhibitor of nuclear factor kappa-B kinase subunit gamma (IKK-γ) is a subunit of the IκB kinase complex that activates NF-κB. NEMO (IKK-γ) is the regulatory subunit of the inhibitor of IκB kinase (IKK) complex, which activates NF-κB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways.

Images

01zGepC0e1ARps3.jpg

WB analysis of IKK gamma/NEMO(GB11628).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

02DdEr176CQEAyl.jpg

WB analysis of IKK gamma/NEMO(GB11628).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.15% ProClin300, 100 µg/mL BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

Datasheet: Download datasheet 1

: AO-03-GB11628-100
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