Français
English
Antibody Product Overview
| ELK.No | ES3249 |
| Product name | PP2Cκ rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | PPM1K; PP2CM; Protein phosphatase 1K; mitochondrial; PP2C domain-containing protein phosphatase 1K; PP2C-like mitochondrial protein; PP2C-type mitochondrial phosphoprotein phosphatase; PTMP; Protein phosphatase 2C isoform kappa; PP2C-kappa |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 152926 |
| Human Swiss-Prot | Q8N3J5 |
| Source | Rabbit |
| Isotype | IgG |
| Target | PP2Cκ |
| Fields | |
| Gene name | PPM1K |
| Protein name | Protein phosphatase 1K mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q8BXN7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PPM1K. AA range:205-254 |
| Specificity | PP2Cκ Polyclonal Antibody detects endogenous levels of PP2Cκ protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 45kD |
| Background | This gene encodes a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. The encoded protein, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore. [provided by RefSeq, Sep 2012], |
| Function | catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,caution:Ref.9 has crystallized PPM1K in the presence of magnesium ions. However, PubMed:17336929 reported that no activity toward p-nitrophenylphosphate was seen in the absence of manganese ions and magnesium could not subsitute for manganese.,cofactor:Binds 1 magnesium or manganese ion per subunit.,function:Regulates the mitochondrial permeability transition pore and is essential for cellular survival and development.,similarity:Belongs to the PP2C family.,similarity:Contains 1 PP2C-like domain., |
| Subcellular location | Mitochondrion matrix . |
| Expression | Amygdala,Cerebellum,Lymph node,Pancreas,Testis, |
Additional Images
No image
Western Blot analysis of various cells using PP2Cκ Polyclonal Antibody
No image
Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using PPM1K Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Western blot analysis of lysates from 293 cells, using PPM1K Antibody. The lane on the right is blocked with the synthesized peptide.
No image
Western blot analysis of the lysates from HeLa cells using PPM1K antibody.
: AO-06-ES3249-100
: 10 Items
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package