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Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
AO-06-ELK0694-48T
Product Overview
| Cat.No | ELK0694 |
| Product Name | Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit |
| Reactivity | Human |
| Alternative Names | 25 Hydroxy Vitamin D; |
| Assay Type | Competitive Inhibition |
| Sensitivity | 1.45 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2.5h |
| Research Area | Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK0694-48T
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