Français
English
Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
AO-06-ELK0694-48T
Product Overview
| Cat.No | ELK0694 |
| Product Name | Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit |
| Reactivity | Human |
| Alternative Names | 25 Hydroxy Vitamin D; |
| Assay Type | Competitive Inhibition |
| Sensitivity | 1.45 ng/mL |
| Standard | 200 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Type | serum, plasma and other biological fluids |
| Assay Length | 2.5h |
| Research Area | Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism; |
| Test Principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
: AO-06-ELK0694-48T
: 10 Produits
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package