Anti-Osteopontin Rabbit pAb
|
Protein full name |
Osteopontin |
|
Synonyms |
SPP1, BNSP, BSPI, ETA-1, OPN, secreted phosphoprotein 1, Osteopontin |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Osteopontin |
|
Uniprot ID |
P10923 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Secreted |
Applications
|
IHC/IF |
Human, Mouse, Rat |
1: 500-1: 2000 |
bone |
Background
Osteopontin (OPN), also known as bone sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by theSPP1 gene (secreted phosphoprotein 1).Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite.
Images
|
|
IHC analysis of OPN (GB11500). Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
|
|
IHC analysis of OPN (GB11500). Sample: Rat bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
|
|
IF analysis of OPN (GB11500). Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
|
|
IF analysis of OPN (GB11500). Sample: Rat bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
|
|
IHC analysis of OPN(GB11500).Sample: Human bone,4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201)，98℃，20 min. Primary antibody:1: 100, 4℃ overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
|
|
IHC analysis of OPN(GB11500). Sample: Human bone,4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201)，98℃，20 min. Primary antibody:1: 500, 4℃ overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
|
|
IHC analysis of OPN(GB11500). Sample: Human bone,4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201)，98℃，20 min. Primary antibody:1: 500, 4℃ overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
Français
English
