Product Information
Protein full name | Tumor susceptibility gene 101 protein |
Synonyms | TSG10, TSG101, tumor susceptibility gene 101, VPS23, tumor susceptibility 101, Cell and organelle markers, ESCRT I complex subunit TSG101, Exosome marker |
Uniprot ID | Q61187 |
Immunogen | KLH conjugated Synthetic peptide corresponding to Human TSG101 |
Predicted MW./Observed MW. | 44 kDa / 46 kDa |
Purity | Affinity purification |
conjugation | HRP |
Applications
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse, Rat | 1: 500-1: 1000 | heart, brain |
| Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-TSG101 antibody(ZB150022-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).
Images
| WB analysis of TSG101(ZB150022-HRP). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Antibody: HRP conjugated anti-TSG101 antibody 1: 500, 4℃ overnight. |
Storage
| Storage | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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