Recombinant Anti-TSG101 antibody (HRP Conjugated)

Recombinant Anti-TSG101 antibody (HRP Conjugated)

AO-03-ZB150022-HRP-100
TSG10,TSG101,tumor susceptibility gene 101,VPS23,tumor susceptibility 101,Cell and organelle markers,ESCRT I complex subunit TSG101,Exosome marker
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Product Information


Protein full name

Tumor susceptibility gene 101 protein

Synonyms

TSG10, TSG101, tumor susceptibility gene 101, VPS23, tumor susceptibility 101, Cell and organelle markers, ESCRT I complex subunit TSG101, Exosome marker

Uniprot ID

Q61187

Immunogen

KLH conjugated Synthetic peptide corresponding to Human TSG101

Predicted MW./Observed MW.

44 kDa / 46 kDa

Purity

Affinity purification

conjugation

HRP




Applications


ApplicationsSpeciesDilutionPositive Tissue
WBMouse, Rat1: 500-1: 1000heart, brain
Incubation conditionAfter blocking, incubate the membrane with HRP conjugated Anti-TSG101 antibody(ZB150022-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.




Background


Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).




Images

01HC0svVjnuMsBn.jpg                        

WB analysis of TSG101(ZB150022-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-TSG101 antibody 1: 500, 4℃ overnight.




Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. 


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).


: AO-03-ZB150022-HRP-100
: 10 Produits
Hurry! only 10 items left in stock.

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