Anti-PCNA antibody (HRP conjugated)

Anti-PCNA antibody (HRP conjugated)

Antibodies-Oncology
AO-03-ZB12010-HRP-100
PCNA,ATLD2,proliferating cell nuclear antigen,Proliferating cell nuclear antigen
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Product Information


Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antigen

Uniprot ID

P17918

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse PCNA

Predicted MW./Observed MW.

29 kDa / 36 kDa

Purity

Affinity purification

conjugation

HRP



Applications


ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000C2C12, testis, placenta
Incubation conditionAfter blocking, incubate the membrane with HRP conjugated Anti-PCNA antibody (ZB12010-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background


Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.



Images

01mDpwJgFqOQSlO.jpg                        

WB analysis of PCNA(ZB12010-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-PCNA antibody 1: 500, 4℃ overnight.

02BZRurbZNMdhQw.jpg                        

WB analysis of PCNA(ZB12010-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-PCNA antibody 1: 500, 4℃ overnight.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. 


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB12010-HRP-100
: 10 Items
Hurry! only 10 items left in stock.

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