Anti-PCNA antibody (HRP conjugated)

Anti-PCNA antibody (HRP conjugated)

Antibodies-Oncology
AO-03-ZB12010-HRP-100
PCNA,ATLD2,proliferating cell nuclear antigen,Proliferating cell nuclear antigen
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Ajouter souhaits

Product Information


Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antigen

Uniprot ID

P17918

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse PCNA

Predicted MW./Observed MW.

29 kDa / 36 kDa

Purity

Affinity purification

conjugation

HRP



Applications


ApplicationsSpeciesDilutionPositive Tissue
WBHuman, Mouse, Rat1: 500-1: 1000C2C12, testis, placenta
Incubation conditionAfter blocking, incubate the membrane with HRP conjugated Anti-PCNA antibody (ZB12010-HRP) for 3-4 hours at room temperature (20-25℃) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST.



Background


Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.



Images

01mDpwJgFqOQSlO.jpg                        

WB analysis of PCNA(ZB12010-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-PCNA antibody 1: 500, 4℃ overnight.

02BZRurbZNMdhQw.jpg                        

WB analysis of PCNA(ZB12010-HRP).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Antibody: HRP conjugated anti-PCNA antibody 1: 500, 4℃ overnight.



Storage


Storage            Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. The color of the product is light yellow. 


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.


Antibodies-Oncology
: AO-03-ZB12010-HRP-100
: 10 Produits
Hurry! only 10 items left in stock.

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