Recombinant Anti-phospho-STAT1 (S727) antibody (Rabbit mAb)

Protein full name

Signal transducer and activator of transcription 1-alpha/beta

Synonyms

STAT1, p-STAT1, p-stat1

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human STAT1 (S727)

Uniprot ID

P42224

Isotype

IgG

Clone Number

SB520

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW. / Observed MW.

87 kDa / 87 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000-1: 2000

HeLa

IHC/IF

Human, Mouse, Rat

1: 4000-1: 8000

colon cancer, esophageal carcinoma, heart

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors.Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus.

Images

WB analysis of phospho-STAT1 (S727)(GB150117).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of phospho-STAT1 (S727)(GB150117).
Sample: Human colon cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Primary antibody: 1: 8000, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of phospho-STAT1 (S727)(GB150117)red.
Sample: Mouse heart, 4% PFA (G1101) 12-24h.
Antigen retrieval: Tris-EDTA buffer (pH 9.0) (G1203), 100℃ 25min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 4000, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.