Anti-Vimentin Rabbit pAb, Primary Antibody
Anti-Vimentin Rabbit pAb
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Description |
Vimentin rabbit polyclonal antibody |
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Protein full name |
Vimentin |
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Synonyms |
VIM, CTRCT30, HEL113, vimentin |
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Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse vimentin |
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Isotype |
IgG |
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Purity |
Affinity purification |
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Subcellular location |
Cytoplasm |
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Predicted MW. |
57 kDa |
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Observed MW. |
57 kDa |
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Uniprot ID |
P08670,P20152,P31000 |
|
reactivity （External validation） |
Pelodiscus sinensis |
Applications
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Applications |
Species |
Dilution |
Positive Sample |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
lung |
|
IHC/IF |
Human, Mouse, Rat |
1: 200-1: 1000 |
colon, lung cancer, ovarian cancer |
|
ICC/IF |
Human, Mouse |
1: 200-1: 1000 |
HeLa cell, NIH/3T3 cell |
Background
Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
Images
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Western blot analysis of Vimentin (GB11192) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human colon using vimentin (GB11192) at dilution of 1: 500 (400x lens) |
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Immunohistochemistry of paraffin embedded human breast cancer using vimentin (GB11192) at dilution of 1: 500 (200x lens) |
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Immunohistochemistry of paraffin embedded human breast cancer using vimentin (GB11192) at dilution of 1: 400 (400x lens) |
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Immunofluorescence of paraffin embedded human colon using vimentin (GB11192) at dilution of 1: 500 (200x lens) |
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Immunofluorescence of paraffin embedded human ovarian cancer using vimentin (GB11192) at dilution of 1: 400 (200x lens) |
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Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cell using Vimentin (GB11192) at dilution of 1: 800 |
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Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH/3T3 cell using Vimentin (GB11192) at dilution of 1: 800 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
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