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Anti-Caspase-3 Rabbit pAb, Primary Antibody
AO-03-GB11767C-50
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Anti-Caspase-3 Rabbit pAb
|
Protein full name |
Caspase-3 |
|
Synonyms |
Casp3, CASP3, CPP32, CPP32B, SCA-1, Caspase 3 |
|
Immunogen |
Recombinant protein corresponding to Mouse Caspase-3 |
|
Uniprot ID |
P70677 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. / Observed MW. |
31 kDa / 17/35 kDa |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Mouse, Rat |
1: 500-1: 1000 |
liver, lung, brain |
Background
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Images
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WB analysis of Caspase-3(GB11767C). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
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WB analysis of Caspase-3(GB11767C). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
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WB analysis of Caspase-3(GB11767C). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
: AO-03-GB11767C-50
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