Recombinant Anti-Phospho-Tau(S202/T205) antibody (Rabbit mAb)

Protein full name

Microtubule-associated protein tau

Synonyms

MAPTL, MTBT1, TAU, MAPT, p-TAU

Immunogen

Recombinant protein corresponding to Human Phospho-Tau(S202/T205)

Uniprot ID

P10636

Isotype

IgG, κ

Clone Number

SB372

Purity

Affinity purification

Subcellular location

Cytoplasm, Cell membrane

Predicted MW. / Observed MW.

79 kDa / 50-80 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000-1: 2000

Cerebral cortex, brain, Alzheimerdiseasebrain

IHC/IF

Human, Mouse, Rat

1: 150-1: 500

colon, brain, Alzheimer's brain

Storage

Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.

background

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity.The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both.Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Images

WB analysis of Phospho-Tau(S202/Y205)(GB150060).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 5000, RT, 1h.

IHC analysis of Phospho-Tau(S202/Y205)(GB150060).
Sample: Rat brain, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 150, 4℃ overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG (H+L), Ready to use(G1302), RT, 20min.

IF analysis of Phospho-Tau(S202/Y205)(GB150060)red.
Sample: Human colon, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 150, 4℃ overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.