S-Vision Poly-HRP Conjugated Goat Anti-Rabbit and Mouse IgG (H+L)
Product Information
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Product Name |
Cat. No. |
Spec. |
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S-Vision Poly-HRP Conjugated Goat Anti-Rabbit and MouseIgG (H+L),Ready to Use |
AO-03-G1303-10ML |
10mL |
|
AO-03-G1303-100ML |
100mL |
Product Description
This productutilize a dextran backbone towhichmultiple HRP enzyme and antibodies molecules are conjugated. This polymer creates a highly sensitive,readily available and one-step non-biotin detection system for immunohistochemical and immunocytochemical staining.This system avoids the use of streptavidin and biotin,and therefore eliminates non-specific staining as a result of endogenous biotin..It has a wide adaptability to sample processing, reducing the variability caused by different tissue processing methods.The reagent is ready-to-use, which does not need to be diluted to obtain the best dyeing ratio, reducing experimental operation errors.
Storage and Shipping Conditions
Wet ice packs for transportation,store at2-8℃ for 12 months.
ProductContent
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Component Number |
Component |
AO-03-G1303-10ML |
AO-03-G1303-100ML |
|
AO-03-G1303 |
S-Vision Poly-HRP Conjugated Goat Anti-Rabbit and MouseIgG (H+L),Ready to Use |
10mL |
100mL |
|
Product Manual |
1份 |
||
Staining Process
1. Manually IHCStaining
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Procedures |
Reagent |
Protocol |
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Dewax |
BioDewax and Clear Solution (RecommendationG1128) |
The sections were placed in BioDewax and Clear SolutionⅠ10min-BioDewax and Clear Solution Ⅱ10min-BioDewax and Clear Solution Ⅲ10min-absolute ethanolⅠ5min-absolute ethanolⅡ5min-absolute ethanol Ⅲ 5min-wash inpure water. |
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Antigen Retrieval |
Citrate AntigenRetrieval Solution pH6.0(RecommendationG1202)orEDTA Antigen Retrieval Solution pH 9.0 (RecommendationG1203) |
Microwave Heating: Heating to boiling,maintain a temperature above 98℃ for 15-20 min,and cooling to room temperature; Pressurecooker heating: Heat in the pressure cooker to air injection for 2 min; Note: The repair time is also related to the fixed time and the antigen epitope<span style="margin:0px;padding:0px;list-style:none;font-family:'等线'; |
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