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Human Interleukin-33, IL33 ELISA Kit
AO-06-BPE073-96T
Full product name
Interleukin-33
Code
BPE073
Assay type
Sandwich
Sensitivity
0.29 pg/mL
Detection range
1.56-100 pg/mL
Sample type
Serum, plasma, cell culture supernates
Species
Human
Storage
2-8°C
Assay time
3.5h
Background
Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.
UniProt accession
MASS（Da）
30759
GeneID
90865
Synonyms
C9orf26;IL1F11;NFHEV
Gene names
IL33
Research area
Cardiovascular,Immunology
Target protein
IL-33
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of BPE073 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 100 pg/ml | 2.339 | 2.308 | 2.199 |
| 2.278 | |||
| 50 pg/ml | 1.342 | 1.365 | 1.256 |
| 1.388 | |||
| 25 pg/ml | 0.744 | 0.768 | 0.659 |
| 0.792 | |||
| 12.5 pg/ml | 0.452 | 0.439 | 0.33 |
| 0.426 | |||
| 6.25 pg/ml | 0.253 | 0.27 | 0.161 |
| 0.288 | |||
| 3.125 pg/ml | 0.174 | 0.183 | 0.074 |
| 0.193 | |||
| 1.5625 pg/ml | 0.156 | 0.15 | 0.041 |
| 0.145 | |||
| 0 pg/ml | 0.106 | 0.109 | 0 |
| 0.113 |
Precision
We measured random samples of BPE073 within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 6.5 | 0.18 | 2.8 |
| Intra-assay | 2 | 20 | 32.6 | 1.3 | 3.9 |
| Intra-assay | 3 | 20 | 65.2 | 3.3 | 5 |
| Inter-assay | 1 | 20 | 6.4 | 0.46 | 7.2 |
| Inter-assay | 2 | 20 | 32.4 | 2 | 6.1 |
| Inter-assay | 3 | 20 | 65.5 | 4.7 | 7.2 |
: AO-06-BPE073-96T
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